In the present study, the waste coral was utilized as a source of calcium oxide for transesterification of soybean oil into biodiesel (methyl esters). Characterization results revealed that the main component of the waste coral is calcium carbonate which transformed in to calcium oxide when calcined above 700 °C. The Box-Behnken design of experiment was carried out and the results were analyzed using response surface methodology. Calcination temperature, methanol: soybean oil molar ratio and catalyst concentration were chosen as variables. The methyl ester content (wt. %) was response which must be maximized. A second-order model was obtained to predict methyl ester content as a function of these variables. Each variable was placed in the three low, medium and high levels (calcination temperature of 700, 800, and 900 °C; catalyst concentration of 3, 6 and 9 wt%; methanol to oil ratios of 12:1, 18:1 and 24:1). The optimum conditions from the experiment were found that the calcination temperature of 900 °C, catalyst concentration of 6 wt%, and methanol to oil ratio of 12:1. Under these conditions methyl ester content reached to 100 wt. %. The waste catalyst was capable of being reused up 4 times without much loss in the activity.