Formaldehyde is widely used in chemical manufacturing industry and classified as a human carcinogen. Discharging wastewater containing formaldehyde without treatment can cause serious risk to the water environment. In this study, a formaldehyde-resistant fungal strain was isolated from sewerage of a furniture factory. Isolate strain was identified based on morphological and phylogenetic analyses. Formaldehyde degrading fungus was determined by characterizing the mycelia growth in culture media, formaldehyde resistant, formaldehyde degrading efficiencies and specific enzyme activity involved in formaldehyde removal. Isolate strain HUA was identified as a member of Aspergillus sydowii. The strain HUA showed a growth in the presence of formaldehyde up to 2400 mg l-1 at an optimum temperature of 25°C and optimum pH of 7. The specific activity of formaldehyde dehydrogenase (FADH) and formate dehydrogenase (FDH) could reach up to 5.02 U mg-1 and 1.06 U mg-1, respectively. It indicated that isolated formaldehyde-resistant A. sydowii HUA strain would be potential used for removing formaldehyde from industrial wastewater.