he optimum period for ciliated protozoa colonizing of an artificial substrate, the polyurethane foams have been assessed in a tropical aquatic ecosystem, the Ekozoa stream of the Mfoundi River Basin in Yaounde (Cameroon). 5 days were calculated as the highest period for the biological indicators of pollution to optimally colonize the artificial substrate. This time interval is the same for all the sampling stations assessed from upstream to downstream and the various microhabitats along the water course. The statistical method applied is that of the completely randomized blocks. The colonization of the substrate increases from the first day to the fifth day, before decreasing to the tenth day. The statistical analysis of variance between the maximum day and the other sampling period was significant at 5 % while the calculation of the value between different points of the same station was not significant. The average number of ciliated protozoan ranges from 20 to 23, from upstream to downstream.