Conventional and experimental methods were studied for the remediation of petroleum contaminated sediments from a dam previously used to collect acid run-off from a sulfur mine. The man-made lake had been neutralized, but bentonite rich sediments remained contaminated with very weathered hydrocarbons (sediments with ~50,000-60,000 mg/kg Total Petroleum Hydrocarbons were used in this study). Biostimulation, bioaugmentation (with native microorganisms) and chemico-biological stabilization, all resulted in similar reductions (14-16%) in the TPH concentration over a three month period. The land farming treatments resulted in variable reductions in toxicity, ranging from nil to complete, while the chemico-biological stabilization treatment, not only eliminated acute toxicity but also resulted in a slight stimulation (~103-109%) of the test organism in the bioassay (Microtox). All three treatments reduced polyaromatic hydrocarbons of probable carcinogenicity to below or nearly below the Mexican norms, reduced Toxic Characteristic Leaching Proceedure leachates to <1 mg/L, and left the material in a pH range of 7.0-7.8. The chemico-biological stabilization has the advantage of only requiring initial mixing of the chemical and organic reagents instead of daily aeration, thereby reducing operating costs. This method is also able to treat very difficult sites at low cost, relying on biological humification processes which are accelerated in a humid tropical and semitropical environment. The total unit cost of the chemico-biological stabilization treatment was estimated to be ~60% of that for land farming in the southern Gulf of Mexico region.
Adams, R. H., (2004a). Chemical-biological stabilization
method for treatment of drilling cuttings and hydrocarbon
contaminated soil. 11th. International Environmental Petroleum Conference. International Environmental Petroleum Consortium, (http://ipec.utulsa.edu), Albuquerque, N. M. 11 â€“ 15 October
Adams, R. H., (2004b). Chemical-biological stabilization of
hydrocarbon contaminated soil and drilling cuttings in
tropical Mexico. Land Contam. Reclam., 12 (11), 349-361 (13 Pages), DOI: 10.2462/09670513.647. Abstract | Full Text
Adams, R. H.; Guzman-Osorio, F. J.; Alvarez-Rivera, J. A.;
DomÃnguez-Rodriguez, V. I., (2007). Long term fertility
monitoring of soil treated by the chemical-biological
stabilization method. 14th. International Environmental Petroleum Conference, International Environmental Petroleum Consortium, (http://ipec.utulsa.edu), Houston, Texas, 6-9 Nov.
Adams, S. R. H., Kanga L. K.; GuzmÃ¡n, O. F. J., (2008). Manejo de humedad para la biorremediaciÃ³n de suelo
contaminado con hidrocarburos del petrÃ³leo. Universidad y Ciencia, en revisiÃ³n.
Atlas, R. M., (1986). Biodegradation of hydrocarbons in the
environment: In: Environmental biotechnology, reducing
risks from environmental chemical through biotechnology
(G.S. Omen, Ed.). Plenum Press, New York.
Bartha, R.; El-Din, N. S., (1993). Testing of some assumptions
about biodegradability in soil as measured by carbon dioxide
evolution. Appl. Environ. Microbiol., 59 (4), 1202-1205 (4 Pages)Abstract | Full Text
Bulinc, A. A., (1990). The luminescent bacteria toxicity test:
its potencial as an in vitro alternative. J. Biolum. Chemilum., 5 (2), 71-77 (7 Pages)Abstract
Doherty, F. G., (2001). A reveiw of the microtox toxicity test
system for assessing toxicity of sediments and soils. Water Qual. Res. J. Can., 36 (3), 475-518 (44 Pages)
EPA (1997). Test methods for evaluating solid waste: Physical/
chemical methods. Environmental Protection Agency, Publication No. EPA 530/SW-846.
Jerger, D. E.; Greenwald, B. P.; Meardon, J. A.; Exner, J. H.;
Allen, M. W., (1991). Bioremediation of PCP-and creosotecontaminated
soil and groundwater. 4th. International IGT symposium on gas, oil, and environmental biotechnology. Colorado Springs, CO., 9-11 Dec.
Kross, B. C.; Cherryholmes, K., (1993). Toxicity screening of
sanitary landfill leachates: a comparative evaluation with
Microtox analyses, chemical, and other toxicity screening
methods.. Richardson ML, editor. Ecotoxicology Monitoring.Weinheim FRG and New York: VCH Publishers: , 225-249 (25 Pages)
McMillen, S.; Smart, R.; Bernier, R., (2002). Biotreating E
and P wastes: Lessons learned from 1992-2002. 9th. International Environmental Petroleum Conference. International Environmental Petroleum Consortium, (http:/ /ipec.utulsa.edu), Albuquerque, N.M. 22-25, Oct. Abstract
Overton, E. B.; Henry, C. B. Jr.; Willison, V.; Mendelssohn, I., (1996). Application of MicrotoxTM assay to establish and
evaluate the efficacy of in-situ burning of oiled marshes. Louisiana Oil Spill Coordinatorâ€™s Office/Office of the Governor, Louisiana Applied Oil Spill Research and Development Program, OSRADP Technical Report Series 96.
Pradhan, S. P.; Paterek, J. R.; Liu, B. Y.; Conrad, J. R.;
Srivastava, V. J., (1997). Pilot-scale bioremediation of PAHcontaminated
soils. Appl. Biochem. Biotech., 63 (5), 759-773 (15 Pages)Abstract | Full Text (754 K)
UJAT (2006). Universidad JuÃ¡rez AutÃ³noma de Tabasco
CaracterizaciÃ³n fÃsico-quÃmica y toxicolÃ³gica de la Presa
Agua de Mina y Presa Anexa, Unidad Minera Texistepec:
Sedimentos. Convenio UJAT-Pemex Gas y PetroquÃmica BÃ¡sica PGPB-SGLPB-UJAT.