Effluents from the leather processing generally are discharged into rivers or are used to irrigate farmland. The biogas production from the digestion of sludge produced could be used as alternative sources for energy and power generation. A study was carried out to examine the effects of various electron acceptor amendments on biogas production from Mexican tannery sludge. Two bench scale experiments were performed: The first experiment used different electron acceptors (nitrate, iron and sulfate) and three different levels for each variable (4.5, 9.0 and 13.5 mg/mL); in the second only urea was used as a nitrogen source with three carbon/nitrogen ratios (6.0, 8.0 and 11). It was found that the reactors with nitrate as electron acceptor (4.5 mg/mL) generated the most biogas (200 mL of biogas accumulated in 25 days). Similarly, the reactors with the lower ratio of carbon/nitrogen (6.0) produced larger biogas production (131.50 ± 19.1 mL of biogas) in a shorter period of time (9 days). The results also showed that the addition of glucose did not significantly influence the production of biogas.