Natural rubber (NR), or cis-1,4 poly-isoprene, is one of the most important biopolymers. For almost a 100 years, million tons of NR-derived products have been produced by humankind as it is very elastic in nature. These products are mainly made up of NR, which is obtained from the latex of tree Hevea brasiliensis commonly called rubber tree. After usage of these NR products, the disposal of these products is the worldwide solid waste problem. To reduce this problem, microbial degradation of the product should be developed. Microbial degradation is mainly carried out by various microorganisms such as bacteria and fungi. Rubber-degrading organisms were isolated by soil burial method. In the isolated organism, Penicillium chrysogenum effectively degraded the NR sample, and enzyme responsible for degradation was also studied. The present study has showed that it is possible to use this strain to degrade the NR.