The current study deals with evaluation of biosorpative feasibility for removal of Cu(II) and Zn(II) by hybrid immobilized biosorbent of Pleurotus sajor-caju and Jasmine sambac. Batch adsorption experiments were carried out to assess the effect of different parameters such as pH, initial metal concentration, biomass dose, temperature and time. The biosorption efficiency of Cu(II) and Zn(II) ions for hybrid immobilized biosorbent elevates with rising pH values. The hybrid immobilized biosorbent illustrated the highest biosorption capability at pH 5 for Cu(II), 6 for Zn(II), at 0.05g /100mL dose and 100 mg/L initial metal concentration of both ions.The concentration of metal ions in aqueous phase was analyzed by atomic absorption spectrophotometer. Uptake kinetics followed the pseudo-second-order model and equilibrium was described by Langmuir and Fraundlich isotherms. Adsorption ratios of Cu(II) and Zn(II) were best fitted to Langmuir isotherm. Biosorbent efficiency at different temperatures was checked and best temperature for ion uptake was found to be 30 OC.